We are interested in two processes fundamental to eukaryotic cellular life: mitosis and membrane trafficking. The lab are working to understand how these processes operate at the molecular level in the hope of identifying new strategies to prevent tumour formation and propagation in cancer and to inhibit cellular infection. Steve Royle is a Senior Cancer Research UK Fellow.
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The mitotic spindle is a tiny machine whose purpose is to accurately segregate the chromosomes during cell division. We are trying to understand how the microtubules of the mitotic spindle are stabilised by non-motor proteins, and how stability is altered in cancer.
>> LATEST Sourav worked out why FGFR3-TACC3 gene fusions in bladder cancer cause mitotic problems. | Link
>> LATEST Faye and Tom have used 3D EM and mathematical modelling to look at mitotic spindle organisation. | Link
Clathrin-mediated endocytosis is the main route of entry into mammalian cells and it controls numerous cellular processes from cell motility and signalling to synaptic transmission and organelle identity. We are trying to understand the molecular mechanisms of this process and also how it is controlled by the cell cycle.
>> LATEST Laura triggers endocytosis on-demand | Link
>> LATEST Nick has developed a new tag for CLEM | Preprint
Wood et al. (2017) New tools for “hot-wiring” clathrin-mediated endocytosis with temporal and spatial precision.
J. Cell Biol., 201702188.
Sarkar, Ryan & Royle (2017) FGFR3–TACC3 cancer gene fusions cause mitotic defects by removal of endogenous TACC3 from the mitotic spindle.
Open Biol., 7: 170080.
Nixon et al. (2017) Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis.
J. Cell Sci., 130: 1845-55.
Nixon et al. (2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle.
eLife, 4: e07635.
Gutiérrez-Caballero et al. (2015) TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation.
Biol. Open, 4: 170-9.
Kaur et al. (2014) An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis.
eLife, 3: e00829.
> Full list
> Mitotic spindle stability >> more here
> Mechanisms of clathrin-mediated endocytosis >> more here
> Research facilities in the lab >> more here
> Plain English summaries of our work >> more here
> The funders who make it all possible >> more here
> Our primary research papers >> more here
> Review articles, methods papers and book chapters >> more here
> Preprints >> more here
> Software and code snippets >> more here