We are interested in two processes fundamental to eukaryotic cellular life: mitosis and membrane trafficking. The lab are working to understand how these processes operate at the molecular level in the hope of identifying new strategies to prevent tumour formation and propagation in cancer and to inhibit cellular infection. Steve Royle is a Senior Cancer Research UK Fellow. >>Recruiting now: JOIN US!!<<
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The mitotic spindle is a tiny machine whose purpose is to accurately segregate the chromosomes during cell division. We are trying to understand how the microtubules of the mitotic spindle are stabilised by non-motor proteins, and how stability is altered in cancer.
>> LATEST Faye and Tom have used 3D EM and mathematical modelling to look at mitotic spindle organisation. | Preprint
>> RECENT Faye showed that K-fibres in the spindle are held together by "The Mesh". | Link
Clathrin-mediated endocytosis is the main route of entry into mammalian cells and it controls numerous cellular processes from cell motility and signalling to synaptic transmission and organelle identity. We are trying to understand the molecular mechanisms of this process and also how it is controlled by the cell cycle.
>> LATEST Laura triggers endocytosis on-demand | Preprint
>> RECENT Clathrin levels and synaptic depression | Link
Nixon et al. (2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle.
eLife, 4: e07635.
Gutiérrez-Caballero et al. (2015) TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation.
Biol. Open, 4: 170-9.
López-Murcia et al. (2014) Presynaptic clathrin levels are a limiting factor for synaptic transmission.
J. Neurosci., 34: 8618-8629.
Kaur et al. (2014) An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis.
eLife, 3: e00829.
Hood et al. (2013) Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding.
J. Cell. Biol., 202: 463-78.
Cheeseman et al. (2013) Specific removal of TACC3–ch-TOG–clathrin at metaphase deregulates kinetochore fiber tension.
J. Cell Sci., 126: 2102-13.
Willox & Royle (2012) Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval.
Curr. Biol., 22: 1435-9.
> Full list
> Mitotic spindle stability >> more here
> Mechanisms of clathrin-mediated endocytosis >> more here
> Research facilities in the lab >> more here
> Plain English summaries of our work >> more here
> The funders who make it all possible >> more here
> Our primary research papers >> more here
> Review articles, methods papers and book chapters >> more here
> Preprints >> more here
> Software and code snippets >> more here