publications

 

  1. papers
  2. preprints
  3. reviews
  4. other
  5. code
  6. covers
  7. altmetrics

Research Papers


> Nixon*, F.M., Honnor*, T.R., Clarke, N.I., Starling, G.P., Beckett, A.J., Johansen, A.M., Brettschneider, J.A., Prior, I.A. & Royle, S.J. (2017) Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis J. Cell Sci., 130: 1845-55. doi: 10.1242/jcs.203877. | Link

> Auckland, P., Clarke, N.I., Royle, S.J. & McAinsh, A.D. (2017) Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment J. Cell Biol., 216: 1623-39. doi: 10.1083/jcb.201607096. | Link

> Nixon, F.M., Gutiérrez-Caballero, C., Hood, F.E., Booth, D.G., Prior, I.A. & Royle, S.J. (2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle eLife, 4: e07635. doi: 10.7554/eLife.07635. | Link | On the cover

> Gutiérrez-Caballero, C., Burgess, S.G., Bayliss, R. & Royle, S.J. (2015) TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation Biol. Open, 4: 170-9. doi: 10.1242/bio.201410843. | Link | On the cover

> López-Murcia, F.J., Royle**, S.J. & Llobet**, A. (2014) Presynaptic clathrin levels are a limiting factor for synaptic transmission J. Neurosci., 34: 8618-8629. | Link | On the cover

> Willox, A.K., Sahraoui, Y.M.E. & Royle, S.J. (2014) Non-specificity of Pitstop 2 in clathrin-mediated endocytosis Biol. Open, 3: 326-31. doi: 10.1242/bio.20147955. | Link

> Kaur, S., Fielding, A.B., Gassner, G., Carter, N.J. & Royle, S.J. (2014) An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis eLife 3: e00829 | Link | PDF

> Hood, F.E., Williams, S.J., Burgess, S.G., Richards, M.W., Roth, D., Straube, A., Pfuhl, M., Bayliss**, R. & Royle**, S.J. (2013) Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding. J. Cell Biol. 202: 463-78. | Link | PDF

> Cheeseman, L.P., Harry, E.F., McAinsh, A.D., Prior, I.A., Royle, S.J. (2013) Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. J. Cell Sci. 126: 2102-13. | Link | PDF | SI

> Willox, A.K. & Royle, S.J. (2012) Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval. Curr. Biol., 22: 1435-9. | Link | PDF | On the cover

> Fielding, A.B., Willox, A.K., Okeke, E. & Royle, S.J. (2012) Clathrin-mediated endocytosis is inhibited during mitosis. Proc. Natl. Acad. Sci. U. S. A., 109: 6572-7. | Link | PDF | On the cover

> Willox, A.K. & Royle, S.J. (2012) Functional analysis of interaction sites on the N-terminal domain of clathrin heavy chain. Traffic, 13: 70-81. | Link | PDF

> Blixt, M.K.E. & Royle, S.J. (2011) Clathrin heavy chain gene fusions expressed in human cancers: analysis of their cellular functions. Traffic, 12: 754-61. | Link | PDF

> Cheeseman, L.P., Booth, D.G., Hood, F.E., Prior, I.A. & Royle, S.J. (2011) Aurora A kinase activity is required for localisation of TACC3/ch-TOG/clathrin inter-microtubule bridges. Commun. Integr. Biol., 4: 409-12. | Link | OA | PDF

> Booth*, D.G., Hood*, F.E., Prior, I.A. & Royle, S.J. (2011) A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. EMBO J., 30: 906-19. | Link | PDF | SI | Rev

> Granseth, B., Odermatt, B, Royle, S.J. & Lagnado, L. (2009) Comment on "The Dynamic Control of Kiss-and-Run and Vesicular Reuse Probed with Single Nanoparticles". Science, 325: 1499. | Link | PDF

> Hood, F.E. & Royle, S.J. (2009) Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis. J. Cell Sci., 122: 2185-90. | Link | PDF | SI

> Granseth*, B., Odermatt*, B., Royle, S.J. & Lagnado, L. (2006) Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses. Neuron, 51(6): 773-86. | Link | PDF | SI | Movie

> Royle, S.J. & Lagnado, L. (2006) Trimerisation is important for the function of clathrin at the mitotic spindle. J. Cell Sci., 119 (Pt 19): 4071-8. | Link | PDF | SI

> Royle, S.J., Bright, N.A. Lagnado, L. (2005) Clathrin is essential for the function of the mitotic spindle. Nature, 434: 1152-7. | Link | PDF | SI

> Royle, S.J., Qureshi, O.S., Bobanovic, L.K., Evans, P.R., Owen, D.J. & Murrell-Lagnado, R.D. (2005) Non-canonical YXXGPhi endocytic motifs: recognition by AP2 and preferential utilization in P2X4 receptors. J. Cell Sci., 118 (Pt 14): 3073-80. | Link | PDF | SI

> Royle, S.J., Bobanovic, L.K. & Murrell-Lagnado, R.D. (2002) Identification of a non-canonical tyrosine-based endocytic motif in an ionotropic receptor. J. Biol Chem., 277: 35378-85. | Link | PDF | SI

> Bobanovic, L.K., Royle, S.J. & Murrell-Lagnado, R.D. (2002) P2X receptor trafficking in neurons is subunit specific. J. Neurosci., 22: 4814-24. | Link | PDF

> Royle, S.J., Collins, F.C., Rupniak, H.T., Barnes, J.C. & Anderson, R. (1999) Behavioural analysis and susceptibility to CNS injury of four inbred strains of mice. Brain Res., 816: 337-49. | Pubmed | PDF


Collaborative papers


> Nahorski, M.S., Al-Gazali, L., Hertecant, J., Owen, D.J., Borner, G.H.H., Chen, Y., Benn, C.L., Carvalho, O.P., Shaikh, S.S., Phelan, A., Robinson, M.S., Royle, S.J. & Woods, C.G. (2015) A novel disorder reveals clathrin heavy chain-22 is essential for human pain and touch development. Brain, 138: 2147-60. doi: 10.1093/brain/awv149. | Link

> O'Regan, L., Sampson, J., Richards, M.W., Knebel, A., Roth, D., Hood, F.E., Straube, A., Royle, S.J., Bayliss, R. & Fry, A.M. (2015) Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression. J. Cell Biol., 209: 349-358. | Link

> Qureshi, O.S., Kaur, S., Hou, T., Jeffery, L.E., Poulter, N.S., Briggs, Z., Kenefeck, R., Willox, A.K., Royle, S.J., Rappoport, J.Z. & Sansom, D.M. (2012) Constitutive clathrin-mediated endocytosis of CTLA-4 persists during T cell activation. J. Biol. Chem., 287: 9429-40. | Link | PMC

 

* = equal contribution
** = joint corresponding authors

Preprints


> Clarke, N.I. & Royle, S.J. (2016) FerriTag: A Genetically-Encoded Inducible Tag for Correlative Light-Electron Microscopy bioRxiv, doi: 10.1101/095208. | Link 18 Dec 2016
> Nixon*, F.M., Honnor*, T.R., Starling, G.P., Beckett, A.J., Johansen, A.M., Brettschneider, J.A., Prior, I.A. & Royle, S.J. (2016) Microtubule organization within mitotic spindles revealed by serial block face scanning EM and image analysis bioRxiv, doi: 10.1101/087866. | Link 15 Nov 2016 | Published in J Cell Sci 15 May 2017
> Wood, L.A., Clarke, N.I., Sarkar, S. & Royle, S.J. (2016) Hot-wiring clathrin-mediated endocytosis in human cells bioRxiv, doi: 10.1101/061986. | Link 4 Jul 2016
> Gutiérrez-Caballero, C., Burgess, S.G., Bayliss, R. & Royle, S.J. (2014) TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation bioRxiv, doi: 10.1101/008359. | Link 21 Aug 2014 | Published in Biol Open 16 Jan 2015
> Willox, A.K., Sahraoui, Y.M.E. & Royle, S.J. (2014) Non-specificity of Pitstop 2 in clathrin-mediated endocytosis bioRxiv, doi: 10.1101/002675. | Link 13 Feb 2014 | Published in Biol Open 4 Apr 2014
> Kaur, S., Fielding, A.B., Gassner, G., Carter, N. J. & Royle, S.J. (2014) An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis bioRxiv, doi: 10.1101/001701. | Link 09 Jan 2014 | Published in eLife 18 Feb 2014

 

* = equal contribution

Reviews


> Wood, L.A. & Royle, S.J. (2015) Zero Tolerance: Amphipathic Helices in Endocytosis Dev. Cell, 33: 119-20. | Link

> Royle, S.J. (2015) Super-duper resolution imaging of mitotic microtubules Nat. Rev. Mol. Cell Biol., 16: 67. | Link

> Royle, S.J. (2013) Protein adaptation: mitotic functions for membrane trafficking proteins Nat. Rev. Mol. Cell Biol., 14: 592-9. | Link | PDF

> Fielding, A.B. & Royle S.J. (2013) Mitotic inhibition of clathrin-mediated endocytosis. Cell Mol. Life Sci., 70: 3423-33. | Link | PDF

> Royle, S.J. (2012) The role of clathrin in mitotic spindle organisation. J. Cell Sci., 125: 19-28. | Link | PDF

> Hood, F.E. & Royle, S.J. (2011) Pulling it together: the role of TACC3 in mitosis. BioArchitecture, 1: 105-9. | Link | PDF

> Royle, S.J. (2011) Mitotic moonlighting functions for membrane trafficking proteins. Traffic, 12: 791-8. | Link | PDF

> Royle, S.J. & Lagnado, L. (2010) Clathrin-mediated endocytosis at the synaptic terminal: bridging the gap between physiology and molecules. Traffic, 11: 1489-97. | Link | PDF | On the cover

> Granseth, B., Odermatt, B., Royle, S.J. & Lagnado, L. (2007) Clathrin-mediated endocytosis: the physiological mechanism of vesicle retrieval at hippocampal synapses. J. Physiol., 585: 681-6. | Link | PDF

> Royle, S.J. (2006) The cellular functions of clathrin. Cell Mol. Life Sci., 63 (16): 1823-32. | Link | PDF

> Royle, S.J. & Lagnado, L. (2003) Endocytosis at the synaptic terminal. J. Physiol., 553: 345-55. | Link | PDF

> Royle, S.J. & Murrell-Lagnado, R.D. (2003) Constitutive cycling: a general mechanism to regulate cell surface proteins. Bioessays, 25: 39-46. | Link | PDF


Methods and Book Chapters


> Booth*, D.G., Cheeseman*, L.P., Prior**, I.A. & Royle**, S.J. (2013) Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy. Methods Cell Biol., 115: 327-42. | Link | PDF

> Royle, S.J. Granseth, B., Odermatt, B., Derevier, A. & Lagnado, L. (2008) Imaging pHluorin-based probes at hippocampal synapses. Methods Mol. Biol., 457: 293-303. | Link | PDF

 

* = equal contribution
** = joint corresponding authors

Other publications


For the latest publications and citations:

> Search for Steve's papers on Pubmed
> Steve's Google Scholar Profile
> ORCID
> Europe PMC
> Scopus profile

About general science


> Team building: Morale boosters by Kendall Powell (2016) Nature
A piece in Nature (NatureJobs) on how to boost lab morale. Steve was interviewed for this piece along with Others from CMCB.

> The Waiting Game by Kendall Powell (2016) Nature
This fantastic article in Nature focused on publication delays. Steve was interviewed for this piece because of his analysis at quantixed (1, 2, 3) which also featured in the article. It was published in February in the run up to the #ASAPbio meeting in the USA which ignited the discussion surrounding the use of preprints in biology to speed up the communication of research.

About our work


> Nixon et al. (2015) eLife
Highlighted by Ryoma Ohi in F1000 Prime. Nice article about "the mesh" in Biotechniques by Kristie Nybo. Overview of the work at serious-science.org. Warwick's press release is here. Lots of syndicated coverage for this paper at various online science websites.

> Willox et al. (2014) Biol Open
Highlighted by Martin Lowe in F1000 Prime.

> Kaur et al. (2014) eLife
Editor's Choice in Cell Biology at The Scientist. A nice feature written by Anna Azvolinsky. Highlights by Kathryn Ayscough and Michael Roth in F1000 Prime. Syndicated coverage of this paper was featured by several outlets including Warwick Medical School, Phys Org, Eurekalert! and BBSRC.

> Hood et al. (2013) J Cell Biol
This paper was covered by Cancer Research UK, the CR-UK science blog, a post at The Conversation and by University of Warwick.

> Cheeseman et al. (2013) J Cell Sci
First author Liam won the 2013 J Cell Sci Award from this paper. Read all about it here. He also won the prize for Young British Cell Biologist of the Year from BSCB for this work. This paper was featured in a Nature News piece by Kendall Powell (Waiting Game) on the delays in scientific publishing.

> Willox & Royle (2012) Curr Biol
Coverage of this paper at University of Liverpool.

> Fielding et al. (2012) PNAS USA
Coverage of this paper at University of Liverpool and BBSRC.

> Willox & Royle (2012) Traffic
Traffic interchange article covering this paper and related work.

> Granseth et al. (2006) Neuron
Preview in Neuron by LoGiudice and Matthews. Faculty of 1000 reviews. Neuron started a 'forum' feature, with this paper as its first (now deleted by Cell Press). Here it is preserved for posterity.

> Royle et al. (2005) Nature
Spindly Clathrin - Nature Structural & Molecular Biology


Some useful code can be found below. These are either code snippets for IgorPro (WaveMetrics) or for ImageJ/FIJI. Our code snippets can also be found on GitHub, IgorExchange and sometimes at quantixed.

>FerriTag | link
Igor Procedures to model FerriTagging, do nanoscale mapping, SNR analysis and more!

>VolumeFinder | link
Igor Procedures for spatial statistics of 3View datasets

>CellMigration | link
Igor Procedures to analyse 2D cell migration. An early version of this code was used in Gutiérrez-Caballero et al., 2015.

>ImageJ Macros | link
ImageJ macros to make Figures with montages. Blind Analysis and more. Add the quantixed ImageJ update site to your ImageJ or FIJI installation.

>Source Code for Nixon et al. 2015 | link | TomogramAnalysis
Several procedures to handle 3D point sets of microtubule positions and to do spatial statistical analysis.

>AvgWaves.ipf | link
A procedure to work out the average point value for each wave in a bunch of 1D waves.

>ViolinPlots.ipf | link
A macro to create a Violin Plot in Igor Pro.

>Map of Igor Functions | code is here
A map of functions is here.

Journal covers

BiOThe K-fiber mesh in three dimensions

Nixon, F.M. et al (2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle,
eLife, doi: 10.7554/eLife.07635.

 

| Paper | Picture

BiOPlus tips of microtubules

Gutiérrez-Caballero, C., Burgess, S.G., Bayliss, R. & Royle, S.J. (2015)
TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation,
Biol. Open, 4: 170-9.

 

| Paper | About

JNClathrin and presynaptic function

López-Murcia, F.J., Royle, S.J. & Llobet, A. (2014)
Presynaptic clathrin levels are a limiting factor for synaptic transmission,
J. Neurosci. 34: 8618-8629.

 

| Paper | About

NRMCBMoonlighting in mitosis

Royle, S.J. (2013)
Protein adaptation: mitotic functions for membrane trafficking proteins,
Nature Rev. Mol. Cell Biol., 14: 592-9.

 

| Pubmed | Paper

CurrBiolClathrin adaptors at the synapse

Willox, A.K. & Royle, S.J. (2012)
Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval.
Curr. Biol., 22: 1435-9.

 

| Pubmed | Paper | Picture

PNASCell division halts endocytosis

Fielding, A.B., Willox, A.K., Okeke, E. & Royle, S.J. (2012)
Clathrin-mediated endocytosis is inhibited during mitosis.
Proc. Natl. Acad. Sci. U. S. A. 109: 6572-7.

 

| Pubmed | Paper | Picture

TrafficTraffic at the synapse

Royle, S.J. & Lagnado, L. (2010)
Clathrin-mediated endocytosis at the synaptic terminal: bridging the gap between physiology and molecules.
Traffic, 11: 1489-97.

 

| Pubmed | Paper | Picture

> Nixon*, F.M., Honnor*, T.R., Clarke, N.I., Starling, G.P., Beckett, A.J., Johansen, A.M., Brettschneider, J.A., Prior, I.A. & Royle, S.J. (2017) Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis J. Cell Sci., 130: 1845-55. doi: 10.1242/jcs.203877
> Nixon, F.M., Gutiérrez-Caballero, C., Hood, F.E., Booth, D.G., Prior, I.A. & Royle, S.J. (2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle eLife, 4: e07635. doi: 10.7554/eLife.07635
> Gutiérrez-Caballero, C., Burgess, S.G., Bayliss, R. & Royle, S.J. (2015) TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biol. Open, 4: 170-9. doi: 10.1242/bio.201410843
> López-Murcia, F.J., Royle**, S.J. & Llobet**, A. (2014) Presynaptic clathrin levels are a limiting factor for synaptic transmission J. Neurosci., 34: 8618-8629. doi: 10.1523/JNEUROSCI.5081-13.2014
> Willox, A.K., Sahraoui, Y.M.E. & Royle, S.J. (2014) Non-specificity of Pitstop 2 in clathrin-mediated endocytosis. Biol. Open doi: 10.1242/bio.20147955
> Kaur, S., Fielding, A.B., Gassner, G., Carter, N. J. & Royle, S.J. (2014) An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis. eLife 3: e00829
> Hood, F.E., Williams, S.J., Burgess, S.G., Richards, M.W., Roth, D., Straube, A., Pfuhl, M., Bayliss**, R. & Royle**, S.J. (2013) Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding. J. Cell Biol. 202:463-78
> Royle, S.J. (2013) Protein adaptation: mitotic functions for membrane trafficking proteins Nature Rev. Mol. Cell Biol., 14: 592-9
> Cheeseman, L.P., Harry, E.F., McAinsh, A.D., Prior, I.A., Royle, S.J. (2013) Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. J. Cell Sci. 126: 2102-13
> Fielding, A.B. & Royle S.J. (2013) Mitotic inhibition of clathrin-mediated endocytosis. Cell Mol. Life Sci., 70: 3423-33
> Willox, A.K. & Royle, S.J. (2012) Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval. Curr. Biol., 22: 1435-9
> Fielding, A.B., Willox, A.K., Okeke, E. & Royle, S.J. (2012) Clathrin-mediated endocytosis is inhibited during mitosis. Proc. Natl. Acad. Sci. U. S. A., 109: 6572-7
> Royle, S.J. (2012) The role of clathrin in mitotic spindle organisation. J. Cell Sci., 125: 19-28
> Willox, A.K. & Royle, S.J. (2012) Functional analysis of interaction sites on the N-terminal domain of clathrin heavy chain. Traffic, 13: 70-81


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