25 July 2011 | The pdf versions of chapters are linked from pubmed. Please get in touch with the editor to request pdf files per email.

 

4 July 2011 | Final proofs for the book sent to the publisher. Expected publication data is 11th August.

 

June 2011 | The book is available to pre-order from Springer or Amazon.

 

Table of content

1 How to Measure Microtubule Dynamics? - Anne Straube

2 Purification of Tubulin from Porcine Brain - Christopher Gell, Claire T. Friel, Barbara Borgonovo, David N. Drechsel, Anthony A. Hyman, and Jonathon Howard

3 Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe - Douglas R. Drummond, Susan Kain, Anthony Newcombe, Christina Hoey, Miho Katsuki, and Robert A. Cross

4 Generation of Differentially Polyglutamylated Microtubules - Benjamin Lacroix and Carsten Janke

5 Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms - Laurence Lafanechere and Didier Job

6 ab -Tubulin and Microtubule-Binding Assays - Jaime N. Campbell and Kevin C. Slep

7 Analysis of Microtubule Assembly Kinetics Using Turbidimetry - Felicia Gaskin

8 Isolation of Centrosomes from Cultured Cells - Simone Reber

9 Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds - Miho Katsuki, Etsuko Muto, and Robert A. Cross

10 Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy - Ivo A. Telley, Peter Bieling, and Thomas Surrey

11 Force Generation by Dynamic Microtubules In Vitro - Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom

12 Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins - Susanne Bechstedt, Michal Wieczorek, Michael Noujaim, and Gary J. Brouhard

13 Analysing the ATP Turnover Cycle of Microtubule Motors - Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard

14 Cryo-electron Tomography of Microtubules Assembled In Vitro from Purified Components - Frederic M. Coquelle, Sophie Blestel, Claire Heichette, Isabelle Arnal, Charles Kervrann, and Denis Chretien

15 Kinetochore Microtubules, Analyzed by Correlated Light and Immunoelectron Microscopy - Andreas Merdes and Jan De Mey

16 Photoactivatable-GFP-a -Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells - Catarina P. Samora and Andrew D. McAinsh

17 Quantification of Asymmetric Microtubule Nucleation at Subcellular Structures - Xiaodong Zhu and Irina Kaverina

18 Investigating Microtubule Dynamic Instability Using Microtubule-Targeting Agents - Stephane Honore and Diane Braguer

19 Laser Ablation of the Microtubule Cytoskeleton: Setting Up and Working with an Ablation System - Nicola Maghelli and Iva Tolić-Norrelykke

20 Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos - Robin Antrobus and James G. Wakefield

21 Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques - Ka Lou Yu, Nanda Keijzer, Casper C. Hoogenraad, and Anna Akhmanova

 

Chapters by CMCB authors