publications

 

  1. papers
  2. preprints
  3. reviews
  4. other
  5. covers
> Sittewelle, M. & Royle, S.J. (2023)
Passive diffusion accounts for the majority of intracellular nanovesicle transport.
Life Sci Alliance. 7 (1) e202302406. doi: 10.26508/lsa.202302406 | Link
> Küey, C., Sittewelle, M., Larocque, G., Hernández-González, M. & Royle, S.J. (2022)
Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation.
eLife 78929. doi: 10.7554/eLife.78929 | Link
> Ferrandiz, N., Downie, L., Starling, G.P. & Royle, S.J. (2022)
Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes.
J Cell Biol. 221: jcb.202203021. doi: 10.1083/jcb.202203021 | Link | Spotlight | Year in Cell Biology
> Smith, S.M., Larocque, G., Wood, K.M., Morris, K.L., Roseman, A.M., Sessions, R.B., Royle, S.J. & Smith, C.J. (2021)
Multi-modal adaptor-clathrin contacts drive coated vesicle assembly.
EMBO J. e108795. doi: 10.15252/embj.2021108795 | Link
> Larocque, G., Moore, D.J., Sittewelle, M., Kuey, C., Hetmanski, J.H.R, La-Borde, P.J., Wilson, B.J., Clarke, N.I., Caswell, P.T. & Royle, S.J. (2021)
Intracellular nanovesicles mediate α5β1 integrin trafficking during cell migration.
J. Cell Biol. 220: e202009028. doi: 10.1083/jcb.202009028 | Link
> Ryan*, E.L., Shelford*, J., Massam-Wu, T., Bayliss, R. & Royle, S.J. (2020)
Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.
J. Cell Sci. 134(3):jcs255794. doi: 10.1242/jcs.255794 | Link
> Larocque, G., La-Borde, P.J., Clarke, N.I., Carter, N.J. & Royle, S.J. (2020)
Tumor Protein D54 defines a new class of intracellular transport vesicles.
J. Cell Biol. 219: e201812044. doi: 10.1083/jcb.201812044. | Link
> Küey, C., Larocque, G., Clarke, N.I & Royle, S.J. (2019)
Unintended inhibition of protein function using GFP nanobodies in human cells.
J. Cell Sci. 132: jcs234955. doi: 10.1242/jcs.234955. | Link
> Clarke, N.I. & Royle, S.J. (2018)
FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy.
Nat. Commun., 9: 2604. doi: 10.1038/s41467-018-04993-0. | Link
> Burgess, S.G., Mukherjee, M., Sabir, S., Joseph, N., Gutierrez-Caballero, C., Richards, M.W., Huguenin-Dezot, N., Chin, J.W., Kennedy, E.J., Pfuhl, M., Royle, S.J., Gergely, F. & Bayliss, R. (2018)
Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic alpha-helix.
EMBO J, 37, e97902. doi: 10.15252/embj.201797902. | Link
> Wood, L.A., Larocque, G., Clarke, N.I., Sarkar, S. & Royle, S.J. (2017)
New tools for “hot-wiring” clathrin-mediated endocytosis with temporal and spatial precision
J. Cell Biol., 216: 4351-65. doi: 10.1083/jcb.201702188. | Link
> Sarkar, S., Ryan, E.L. & Royle, S.J. (2017)
FGFR3–TACC3 cancer gene fusions cause mitotic defects by removal of endogenous TACC3 from the mitotic spindle
Open Biol., 7: 170080. doi: 10.1098/rsob.170080. | Link
> Nixon*, F.M., Honnor*, T.R., Clarke, N.I., Starling, G.P., Beckett, A.J., Johansen, A.M., Brettschneider, J.A., Prior, I.A. & Royle, S.J. (2017)
Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis
J. Cell Sci., 130: 1845-55. doi: 10.1242/jcs.203877. | Link
> Auckland, P., Clarke, N.I., Royle, S.J. & McAinsh, A.D. (2017)
Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment
J. Cell Biol., 216: 1623-39. doi: 10.1083/jcb.201607096. | Link
> Nixon, F.M., Gutiérrez-Caballero, C., Hood, F.E., Booth, D.G., Prior, I.A. & Royle, S.J. (2015)
The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle
eLife, 4: e07635. doi: 10.7554/eLife.07635. | Link | On the cover
> Gutiérrez-Caballero, C., Burgess, S.G., Bayliss, R. & Royle, S.J. (2015)
TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Biol. Open, 4: 170-9. doi: 10.1242/bio.201410843. | Link | On the cover
> López-Murcia, F.J., Royle**, S.J. & Llobet**, A. (2014)
Presynaptic clathrin levels are a limiting factor for synaptic transmission
J. Neurosci., 34: 8618-8629. | Link | On the cover
> Willox, A.K., Sahraoui, Y.M.E. & Royle, S.J. (2014)
Non-specificity of Pitstop 2 in clathrin-mediated endocytosis
Biol. Open, 3: 326-31. doi: 10.1242/bio.20147955. | Link
> Kaur, S., Fielding, A.B., Gassner, G., Carter, N.J. & Royle, S.J. (2014)
An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis
eLife 3: e00829 | Link | PDF
> Hood, F.E., Williams, S.J., Burgess, S.G., Richards, M.W., Roth, D., Straube, A., Pfuhl, M., Bayliss**, R. & Royle**, S.J. (2013)
Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding.
J. Cell Biol. 202: 463-78. | Link | PDF
> Cheeseman, L.P., Harry, E.F., McAinsh, A.D., Prior, I.A., Royle, S.J. (2013)
Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension.
J. Cell Sci. 126: 2102-13. | Link | PDF | SI
> Willox, A.K. & Royle, S.J. (2012)
Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval.
Curr. Biol., 22: 1435-9. | Link | PDF | On the cover
> Fielding, A.B., Willox, A.K., Okeke, E. & Royle, S.J. (2012)
Clathrin-mediated endocytosis is inhibited during mitosis.
Proc. Natl. Acad. Sci. U. S. A., 109: 6572-7. | Link | PDF | On the cover
> Willox, A.K. & Royle, S.J. (2012)
Functional analysis of interaction sites on the N-terminal domain of clathrin heavy chain.
Traffic, 13: 70-81. | Link | PDF
> Blixt, M.K.E. & Royle, S.J. (2011)
Clathrin heavy chain gene fusions expressed in human cancers: analysis of their cellular functions.
Traffic, 12: 754-61. | Link | PDF
> Cheeseman, L.P., Booth, D.G., Hood, F.E., Prior, I.A. & Royle, S.J. (2011)
Aurora A kinase activity is required for localisation of TACC3/ch-TOG/clathrin inter-microtubule bridges.
Commun. Integr. Biol., 4: 409-12. | Link | OA | PDF
> Booth*, D.G., Hood*, F.E., Prior, I.A. & Royle, S.J. (2011)
A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging.
EMBO J., 30: 906-19. | Link | PDF | SI | Rev
> Granseth, B., Odermatt, B, Royle, S.J. & Lagnado, L. (2009)
Comment on "The Dynamic Control of Kiss-and-Run and Vesicular Reuse Probed with Single Nanoparticles".
Science, 325: 1499. | Link | PDF
> Hood, F.E. & Royle, S.J. (2009)
Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis.
J. Cell Sci., 122: 2185-90. | Link | PDF | SI
> Granseth*, B., Odermatt*, B., Royle, S.J. & Lagnado, L. (2006)
Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses.
Neuron, 51(6): 773-86. | Link | PDF | SI | Movie
> Royle, S.J. & Lagnado, L. (2006)
Trimerisation is important for the function of clathrin at the mitotic spindle.
J. Cell Sci., 119 (Pt 19): 4071-8. | Link | PDF | SI
> Royle, S.J., Bright, N.A. Lagnado, L. (2005)
Clathrin is essential for the function of the mitotic spindle.
Nature, 434: 1152-7. | Link | PDF | SI
> Royle, S.J., Qureshi, O.S., Bobanovic, L.K., Evans, P.R., Owen, D.J. & Murrell-Lagnado, R.D. (2005)
Non-canonical YXXGPhi endocytic motifs: recognition by AP2 and preferential utilization in P2X4 receptors.
J. Cell Sci., 118 (Pt 14): 3073-80. | Link | PDF | SI
> Royle, S.J., Bobanovic, L.K. & Murrell-Lagnado, R.D. (2002)
Identification of a non-canonical tyrosine-based endocytic motif in an ionotropic receptor.
J. Biol Chem., 277: 35378-85. | Link | PDF | SI
> Bobanovic, L.K., Royle, S.J. & Murrell-Lagnado, R.D. (2002)
P2X receptor trafficking in neurons is subunit specific.
J. Neurosci., 22: 4814-24. | Link | PDF
> Royle, S.J., Collins, F.C., Rupniak, H.T., Barnes, J.C. & Anderson, R. (1999)
Behavioural analysis and susceptibility to CNS injury of four inbred strains of mice.
Brain Res., 816: 337-49. | Pubmed | PDF

Collaborative papers


> Nahorski, M.S., Al-Gazali, L., Hertecant, J., Owen, D.J., Borner, G.H.H., Chen, Y., Benn, C.L., Carvalho, O.P., Shaikh, S.S., Phelan, A., Robinson, M.S., Royle, S.J. & Woods, C.G. (2015)
A novel disorder reveals clathrin heavy chain-22 is essential for human pain and touch development.
Brain, 138: 2147-60. doi: 10.1093/brain/awv149. | Link
> O'Regan, L., Sampson, J., Richards, M.W., Knebel, A., Roth, D., Hood, F.E., Straube, A., Royle, S.J., Bayliss, R. & Fry, A.M. (2015)
Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression.
J. Cell Biol., 209: 349-358. | Link
> Qureshi, O.S., Kaur, S., Hou, T., Jeffery, L.E., Poulter, N.S., Briggs, Z., Kenefeck, R., Willox, A.K., Royle, S.J., Rappoport, J.Z. & Sansom, D.M. (2012)
Constitutive clathrin-mediated endocytosis of CTLA-4 persists during T cell activation.
J. Biol. Chem., 287: 9429-40. | Link | PMC

* = equal contribution
** = joint corresponding authors

Preprints


Coming soon...

Preprint only


> Martins Ratamero, E. & Royle, S.J. (2019) Calculating the maximum capacity of intracellular transport vesicles bioRxiv, doi: 10.1101/555813. | Link 20 Feb 2019

Now published


> Sittewelle, M. & Royle, S.J. (2023) Passive diffusion accounts for the majority of intracellular vesicle transport bioRxiv, doi: https://doi.org/10.1101/2023.05.10.540182. | Link 11 May 2023
> Küey, C., Sittewelle, M., Larocque, G., Hernández-González, M. & Royle, S.J. (2022) Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation bioRxiv, doi: https://doi.org/10.1101/2022.03.21.485220. | Link 21 Mar 2022 | Published in eLife 20 Jul 2022
> Ferrandiz, N., Downie, L., Starling, G.P. & Royle, S.J. (2021) Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes bioRxiv, doi: https://doi.org/10.1101/2021.04.23.441091. | Link 23 Apr 2021 | Published in J Cell Biol 6 Jun 2022
> Smith, S.M., Larocque, G., Wood, K.M., Morris, K.L., Roseman, A.M., Sessions, R.B., Royle, S.J. & Smith, C.J. (2021) Multi-modal adaptor-clathrin contacts drive coated vesicle assembly bioRxiv, doi: https://doi.org/10.1101/2021.05.22.445240. | Link 22 May 2021 | Published in EMBO J 6 Sep 2021
> Larocque, G., La-Borde, P.J., Wilson, B.J., Clarke, N.I., Moore, D.J., Caswell, P.T. & Royle, S.J. (2020) Intracellular nanovesicles mediate integrin trafficking during cell migration bioRxiv, doi: https://doi.org/10.1101/2020.08.19.257287. | Link 19 Aug 2020 | Published in J Cell Biol 22 July 2021
> Ryan, E.L., Shelford, J., Massam-Wu, T., Bayliss, R. & Royle, S.J. (2020) Defining endogenous TACC3–chTOG–clathrin–GTSE1 interactions at the mitotic spindle using induced relocalization bioRxiv, doi: https://doi.org/10.1101/2020.07.01.181818. | Link 01 Jul 2020 | Published in J Cell Sci 30 Dec 2020
> Kuey, C., Larocque, G., Clarke, N.I & Royle, S.J. (2019) Unintended inhibition of protein function using GFP nanobodies in human cells bioRxiv, doi: 10.1101/590984. | Link 27 Mar 2019 | Published in J Cell Sci 10 Oct 2019
> Larocque, G., La-Borde, P.J., Clarke, N.I., Carter, N.J. & Royle, S.J. (2019) Tumor Protein D54 defines a new class of intracellular transport vesicle bioRxiv, doi: 10.1101/481754. | Link 28 Nov 2018 | Published in J Cell Biol 31 Oct 2019
> Clarke, N.I. & Royle, S.J. (2016) FerriTag: A Genetically-Encoded Inducible Tag for Correlative Light-Electron Microscopy bioRxiv, doi: 10.1101/095208. | Link 18 Dec 2016 | Published in Nat Commun 04 July 2018
> Nixon*, F.M., Honnor*, T.R., Starling, G.P., Beckett, A.J., Johansen, A.M., Brettschneider, J.A., Prior, I.A. & Royle, S.J. (2016) Microtubule organization within mitotic spindles revealed by serial block face scanning EM and image analysis bioRxiv, doi: 10.1101/087866. | Link 15 Nov 2016 | Published in J Cell Sci 15 May 2017
> Wood, L.A., Clarke, N.I., Sarkar, S. & Royle, S.J. (2016) Hot-wiring clathrin-mediated endocytosis in human cells bioRxiv, doi: 10.1101/061986. | Link 4 Jul 2016 | Published in J Cell Biol 26 Sep 2017
> Gutiérrez-Caballero, C., Burgess, S.G., Bayliss, R. & Royle, S.J. (2014) TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation bioRxiv, doi: 10.1101/008359. | Link 21 Aug 2014 | Published in Biol Open 16 Jan 2015
> Willox, A.K., Sahraoui, Y.M.E. & Royle, S.J. (2014) Non-specificity of Pitstop 2 in clathrin-mediated endocytosis bioRxiv, doi: 10.1101/002675. | Link 13 Feb 2014 | Published in Biol Open 4 Apr 2014
> Kaur, S., Fielding, A.B., Gassner, G., Carter, N. J. & Royle, S.J. (2014) An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis bioRxiv, doi: 10.1101/001701. | Link 09 Jan 2014 | Published in eLife 18 Feb 2014

 

* = equal contribution

Reviews


> Sittewelle, M., Ferrandiz, N., Fesenko, M. & Royle, S.J. (2023)
Genetically encoded imaging tools for investigating cell dynamics at a glance
J. Cell Sci., 136 (7): jcs260783. | Link
> Larocque, G. & Royle, S.J. (2022)
Integrating intracellular nanovesicles into integrin trafficking pathways and beyond
Cell. Mol. Life Sci., 79: 335. | Link
> Shah, P., Chaumet, A., Royle, S.J. & Bard, F.A. (2019)
The NAE Pathway: Autobahn to the Nucleus for Cell Surface Receptors.
Cells, 33: 119-20. | Link
> Wood, L.A. & Royle, S.J. (2015)
Zero Tolerance: Amphipathic Helices in Endocytosis.
Dev. Cell, 33: 119-20. | Link
> Royle, S.J. (2015)
Super-duper resolution imaging of mitotic microtubules.
Nat. Rev. Mol. Cell Biol., 16: 67. | Link
> Royle, S.J. (2013)
Protein adaptation: mitotic functions for membrane trafficking proteins.
Nat. Rev. Mol. Cell Biol., 14: 592-9. | Link | PDF
> Fielding, A.B. & Royle S.J. (2013)
Mitotic inhibition of clathrin-mediated endocytosis.
Cell Mol. Life Sci., 70: 3423-33. | Link | PDF
> Royle, S.J. (2012)
The role of clathrin in mitotic spindle organisation.
J. Cell Sci., 125: 19-28. | Link | PDF
> Hood, F.E. & Royle, S.J. (2011)
Pulling it together: the role of TACC3 in mitosis.
BioArchitecture, 1: 105-9. | Link | PDF
> Royle, S.J. (2011)
Mitotic moonlighting functions for membrane trafficking proteins.
Traffic, 12: 791-8. | Link | PDF
> Royle, S.J. & Lagnado, L. (2010)
Clathrin-mediated endocytosis at the synaptic terminal: bridging the gap between physiology and molecules.
Traffic, 11: 1489-97. | Link | PDF | On the cover
> Granseth, B., Odermatt, B., Royle, S.J. & Lagnado, L. (2007)
Clathrin-mediated endocytosis: the physiological mechanism of vesicle retrieval at hippocampal synapses.
J. Physiol., 585: 681-6. | Link | PDF
> Royle, S.J. (2006)
The cellular functions of clathrin.
Cell Mol. Life Sci., 63 (16): 1823-32. | Link | PDF
> Royle, S.J. & Lagnado, L. (2003)
Endocytosis at the synaptic terminal.
J. Physiol., 553: 345-55. | Link | PDF
> Royle, S.J. & Murrell-Lagnado, R.D. (2003)
Constitutive cycling: a general mechanism to regulate cell surface proteins.
Bioessays, 25: 39-46. | Link | PDF

Methods and Book Chapters


> Ferrandiz, N. & Royle*, S.J. (2023)
3D Ultrastructural Visualization of Mitosis Fidelity in Human Cells Using Serial Block Face Scanning Electron Microscopy (SBF-SEM).
Bio Protoc., 13:e4708. | Link
> Wood, L.A. & Royle*, S.J. (2018)
Imaging "Hot-Wired" Clathrin-Mediated Endocytosis.
Methods Mol. Biol., 1847:83-94. | Link | PDF
> Clarke, N.I. & Royle*, S.J. (2018)
Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture.
Methods Cell Biol., 145: 29-43. | Link | PDF
> Booth*, D.G., Cheeseman*, L.P., Prior**, I.A. & Royle**, S.J. (2013)
Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy.
Methods Cell Biol., 115: 327-42. | Link | PDF
> Royle, S.J. Granseth, B., Odermatt, B., Derevier, A. & Lagnado, L. (2008)
Imaging pHluorin-based probes at hippocampal synapses.
Methods Mol. Biol., 457: 293-303. | Link | PDF

 

* = equal contribution
** = joint corresponding authors
For the latest publications and citations:

> Search for Steve's papers on Pubmed
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Other publications and outputs


DigitalCellThe Digital Cell

A practical guide for cell, developmental and molecular biologists to deal with data.
Published by Cold Spring Harbor Laboratory Press, the book covers programming, statistics, image analysis, data visualisation, electronic lab notebooks and much more.
It's recommended for new research students and for anyone who wants to brush up their quantitative cell biology skills.


>> Royle (2019) | [Buy It Here]



Datasets

> Shelford, J. & Royle, S.J. (2020) Comparing antibodies for the detection of ch-TOG/CKAP5 in mitotic HeLa cells by indirect immunofluorescence. figshare. Figure. doi: https://doi.org/10.6084/m9.figshare.13395554.v1 | Link 17 Dec 2020

Software

> TrackMateR doi: https://doi.org/10.5281/zenodo.453722113 DOI
> CellMigration doi: https://doi.org/10.5281/zenodo.4850277 DOI
> CellShape doi: https://doi.org/10.5281/zenodo.3959278 DOI

Software under public development is usually at Steve's github repo. The lab GitHub and the CMCB GitHub have forks of our popular projects.

Journal covers

JCBChromosome ensheathing

Ferrandiz et al. (2022) Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes,
J Cell Biol, 221 (6): e202203021.
| Paper | Picture

eLifeThe K-fiber mesh in three dimensions

Nixon, F.M. et al (2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle,
eLife, doi: 10.7554/eLife.07635.
| Paper | Picture

BiOPlus tips of microtubules

Gutiérrez-Caballero, C., Burgess, S.G., Bayliss, R. & Royle, S.J. (2015)
TACC3–ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation,
Biol. Open, 4: 170-9.
| Paper | About

JNClathrin and presynaptic function

López-Murcia, F.J., Royle, S.J. & Llobet, A. (2014)
Presynaptic clathrin levels are a limiting factor for synaptic transmission,
J. Neurosci. 34: 8618-8629.
| Paper | About

NRMCBMoonlighting in mitosis

Royle, S.J. (2013)
Protein adaptation: mitotic functions for membrane trafficking proteins,
Nature Rev. Mol. Cell Biol., 14: 592-9.
| Pubmed | Paper

CurrBiolClathrin adaptors at the synapse

Willox, A.K. & Royle, S.J. (2012)
Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval.
Curr. Biol., 22: 1435-9.
| Pubmed | Paper | Picture

PNASCell division halts endocytosis

Fielding, A.B., Willox, A.K., Okeke, E. & Royle, S.J. (2012)
Clathrin-mediated endocytosis is inhibited during mitosis.
Proc. Natl. Acad. Sci. U. S. A. 109: 6572-7.
| Pubmed | Paper | Picture

TrafficTraffic at the synapse

Royle, S.J. & Lagnado, L. (2010)
Clathrin-mediated endocytosis at the synaptic terminal: bridging the gap between physiology and molecules.
Traffic, 11: 1489-97.
| Pubmed | Paper | Picture

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