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The labs core interest is the kinetochore. These are adaptive, multi-layered mechanochemical machines that assemble at the centromere of each sister chromatid and engage on their outer face with the plus ends of k-fibres, microtubule bundles that emanate from the spindle poles.
We envision the kinetochore as a set of interacting springs, clutches and motors and the problem of kinetochore mechanism as one of understanding how these functional modules assemble, disassemble and interact with one another to give rise to the emergent properties of the kinetochore.
Approaches in the McAinsh lab include live-cell microscopy, computational image analysis, mathematical modelling and in vitro reconstitution.
How Ndc80 and CENPO complexes work together in the kinetochores; Experiments by Phil and Muriel and nice collaboration with Musacchio and Raunser labs
>> Pesenti ME, Prumbaum D, Auckland P, Smith CM, Faesen AC, Petrovic A, Erent M, Maffini S, Pentakota S, Weir JR, Lin Y-C,Raunser S, McAinsh AD and Musacchio. Molecular Cell, 2018 |
Here we show using genome editing how BUB1 is not essential for the spindle checkpoint in human cells; collaboration with Millar lab
>> Currie CE, Mora-SantosM, Smith CA, McAinsh AD and Millar JBA. Current Biology, 2018 |
Collaboration with Susana Godinho - includes tracking of centrosome dynamics by Chris
>> Rhys AD, Monteiro P, Smith C, Vaghela M, Arnandis T, Kato T, Leitinger B, Sahai E, McAinsh A, Charras G, Godinho SA. J. Cell Biol, 2017 |
Collaboration with Prasad Jallepalli - includes Hauke's cool in vitro reconstitution of Ska complex on dynamic microtubules.
>> Maciejowski J, Drechsler H, Grundner-Culemann K, Ballister ER, ... McAinsh AD, Jallepalli PV. Dev. Cell. 2017 Dev Cell. 2017 41:143-156 |
Phil Auckland finds that kinetochres mature and this is important to prevent premature detachment fromt he mitoic spindle.
>> Auckland, P., Clarke I., Royle S.J. and McAinsh A.D. J. Cell Biol. 2017 J Cell Biol. 2017 Jun 5;216(6):1623-1639 |
Chris Smith shows how kinetochores are a swivel joint that aids connection to the mitotic spindle.
>> Smith A.C., McAinsh A.D. and Burroughs N.J. eLife 2016 5:e16159 | [Open Access]
Hauke Drechsler reveals that Kif15 is a multi-function motor that can rearrange microtubule networks, track plus-ends and control dynamics.
>> Drechsler H, McAinsh AD. Proc Natl Acad Sci U S A. 2016 113:E1635-44 | [Open Access]
Code and User Guides for our software tools and packages can all be found on our CMCB Github page. These include:
KiT (Tracking kinetochores in microscopy movies in 2D or 3D, and in multiple channels)
SiD (Spot Intensity Detector) - unpublished and please use!