dynein activation on microtubule bundles
We show that human dynein is activated when it crossbridges two microtubules. Dynein polarity-sorts microtubule bundles by selectively sliding anti-parallel microtubules apart.
While single molecules of dynein are predominantly static or diffusive on single microtubules, they walks processively on the microtubule bundles they form. Their force output doubles on microtubule bundles
and we propose a model whereby the separation of the motor domains during crossbridging activates the motor.
Manas Chakraborty, Algirdas Toleikis, Nida Siddiqui, Robert A. Cross, and Anne Straube (2020)
Activation of cytoplasmic dynein through microtubule crossbridging.
PREPRINT bioRxiv 2020.04.13.038950
doi: 10.1101/2020.04.13.038950. [Link]
This preprint has been highlighted by a prelight.
synergy of microtubule-targeting drugs
The screening team at the Beatson Institute identified Mebendazole as a candidate to act in synergy with Docetaxel. We contributed
experiments in RPE1 and PC3 cells treated with each of the drugs individually and together to show how they affect microtubule
assembly and mitotic progression. The synergy of both drugs results frequently in mitotic catastrophe.
Linda Rushworth, Kay Hewit, Sophie Munnings-Tomes, Sukrut Somani, Daniel James, Emma Shanks, Christine Dufès, Anne Straube, Rachana Patel, and Hing Y Leung (2019)
Repurposing screen identifies mebendazole as a clinical candidate to synergise with docetaxel for prostate cancer treatment.
British Journal of Cancer.
doi: 10.1038/s41416-019-0681-5. [Link]
phosphoregulation of microtubule binding
Here we show that EML4 binds microtubules via the negatively charged E-hooks. Upon phosphorylation by mitotic kinases NEK6 and NEK7, EML4 binding to microtubules is reduced. This displacement of EML4 from microtubules reduces their stability. Dynamic microtubules are required for faithful chromosome segregation. Expressing non-phosphorylatable mutants of EML4 results in their association to microtubules throughout mitosis and spindle checkpoint activation and mitotic delay.
Adib R, Montgomery JM, Atherton J, O'Regan L, Richards MW, Straatman KR, Roth
D, Straube A, Bayliss R, Moores CA, Fry AM. (2019)
Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression.
Sci Signal. 12(594):eaaw2939.
microtubules in cell migration
This is our contribution to the special issue on BIOCHEMICAL AND MECHANISTIC ASPECTS OF CELL MOTILITY AND MIGRATION. Our Essay summarises the functions of microtubules in cell migration including its role as directional transport tracks, signalling platforms and load-bearing elements.
Clare Garcin and Anne Straube (2019)
Microtubules in cell migration
Essays in Biochemistry 63(5): 509-520.
activating intracellular transport
We show that contrary to other kinesin-3 motors, KIF1C is autoinhibited by an intramolecular interaction of its stalk with the microtubule binding surface of the motor domain. The phosphatase PTPN21 and the cargo adpater Hook3 activate KIF1C by binding to the stalk. Both activators increase the landing rate of the kinesin and are transported by the motor.
N. Siddiqui*, A.J. Zwetsloot*, A. Bachmann, D. Roth, H. Hussain, J. Brandt, I. Kaverina & A. Straube (2019)
PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport
Nature Communications, Volume 10, Article number: 2693
[Link to Paper]
Siddiqui,N., Bachmann, A., Zwetsloot,A., Hussain,H., Roth, D., Kaverina, I. and Straube, A. (2018)
Activation of intracellular transport by relieving KIF1C autoinhibition.
PREPRINT bioRxiv doi: 10.1101/488049
[Link to Preprint]
This preprint has been highlighted by a prelight.
[Link to Press Release]
spatial EB positioning
Our contribution to the special issue on reconstituting cell biology. We reconstituted the spatially distinct tip tracking of EB proteins in vitro and show that EBs sense the nucleotide state of two the beta-tubulins adjacent to their binding site. EB2 has a different nucleotide preference and prefers binding to mixed nucleotide lattices, while EB1 and EB3 need an environment of pure GTPgS to bind efficiently.
Roth, D., Fitton, B.P., Chmel, N., Wasiluk, N. and Straube, A. (2018)
Spatial positioning of EB family proteins at microtubule tips involves distinct nucleotide-dependent binding properties
Journal of Cell Science 132(4): jcs219550
[link to full pdf]
measuring microtubule dynamics
Our essay on how to measure microtubule dynamics.
Zwetsloot, A.J., Tut, G. and Straube, A. (2018)
Measuring microtubule dynamics
Essays in Biochemistry 62(6):725-735.
[link to open access article]
Nida and Anne review kinesin-3 transporters, their structural characteristics, intracellular cargoes and their regulation, with particular emphasis on the autoinhibited state and mechanisms of relieving autoinhibition by cargo and adapter proteins.
Siddiqui, N. and Straube, A. (2017)
Intracellular cargo transport by kinesin-3 motors
Biochemistry (Moscow), Vol. 82, No. 7, pp. 803–815
Russian version: Biokhimiya, 2017, Vol. 82, No. 7, pp. 1047–1062
[link to open access article]
microtubules regulating cell migration
A comprehensive book chapter in "The Microtubule Cytoskeleton" exploring the diverse functions of microtubules in cell migration and neuronal pathfinding. Co-authored with Ulrike Theisen who is studying neuronal migration in zebrafish in Braunschweig.
Ulrike Theisen and Anne Straube (2016)
Microtubules Regulate Cell Migration and Neuronal Pathfinding
in: The Microtubule Cytoskeleton, Jens Lüders (ed), pages 151-189 DOI: 10.1007/978-3-7091-1903-7_6
Anne's contribution to a major textbook with 285 chapters. My chapter contains all you ever wanted to know about microtubules and the proteins that bind and regulate them in a nutshell.
Straube, A. (2015)
Microtubules and Microtubule-Associated Proteins (MAPs)
Encyclopedia of Cell Biology, Volume 2: Organizational Cell Biology, 2016, Pages 539-547
[link] [request pdf]
wobbly phages as nanosensors
This paper demonstrates the use of a fluorescently-labelled and surface-anchored bacteriophage M13 to measure wall shear stress. Bacteriophage anchored on collagen-coated slides and the surface of endothelial cells were subjected to various levels of shear stress by modulating buffer flow through the imaging chamber.
Lobo, D.P. ,Wemyss, A.M., Smith, D.J., Straube, A., Betteridge, K.B., Salmon, A.H.J., Foster, R.R., Elhegni, H.E., Satchell, S.C., Little, H.A., Pacheco-Gomez, R., Simmons, M.J., Hicks, M.R., Bates, D.O., Rodger, A., Dafforn, T.R., & Arkill, K.P. (2015)
Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle.
Nano Research, doi: 10.1007/s12274-015-0831-x
chaperoning the spindle
We describe Nek6-dependent spindle recruitment of Hsp70, which is required for K-fibre stability and function. Data generated in the Straube lab suggest that the weak direct interaction of Hsp70 with microtubules is not affected by the Nek6-mediated phosphorylation at T66. Thus Hsp70 possibly acts by recruiting the chTOG/TACC3/Clathrin complex to spindle microtubules.
O'Regan, L., Sampson, J., Richards, M.W., Knebel, A., Roth, D., Hood, F.E., Straube, A., Royle, S.J., Bayliss, R. & Fry, A.M. (2015)
Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression.
The Journal of Cell Biology, 209: 349-358. doi:10.1083/jcb.201409151
Here, we identify a previously uncharacterised isoform of microtubule-associated protein MAP4, oMAP4, as a microtubule organising factor that is crucial for myogenesis. Depletion of oMAP4 impairs cell elongation and cell-cell fusion. oMAP4 is required for paraxial microtubule organisation in muscle cells and prevents dynein- and kinesin-driven microtubule-microtubule sliding. Purified oMAP4 aligns dynamic microtubules into antiparallel bundles that withstand motor forces in vitro.
Mogessie, B., Roth, D., Rahil, Z. and Straube, A. (2015)
A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation
eLife, 4: e05697. doi:10.7554/eLife.05697.
[link] | [PDF]
microtubule binding motif revealed
In this paper we identify the MT-binding domain in EML proteins. Daniel and Anne performed TIRF-based MT binding assays using full-length, mutant and truncation constructs of human EML1 fused to YFP. We show that EML contains a trimerisation domain that is required, but not sufficient for MT binding.
Richards, M.W., O'Regan, L., Roth, D., Montgomery, J.M., Straube, A., Fry, A.M. and Bayliss, R. (2015)
Microtubule association of EML proteins and the EML4-ALK variant 3 oncoprotein require an N-terminal trimerization domain
Biochemical Journal, 467: 529-536. doi:10.1042/BJ20150039
transport on the go
Alice and Anne review the literature implicating kinesins in cell migration. It turns out we know surprisingly little about which kinesin transports which cargo to support cell polarity and directed migration. What we do know, you'll find in here.
Bachmann, A. and Straube, A. (2015)
Kinesins in cell migration
Biochem Soc Trans, 43(1): 79-83.
[PDF] | [pubmed]
kinesin under MAP control
Here we show that the kinesin KIF1C is required for the de-novo formation of podosomes in vascular smooth muscle cells. We describe a pathway whereby activation of PKC results in the enrichment of CLASPs at microtubule ends, which stimulates KIF1C translocation to the cell periphery, where KIF1C localises to incipient podosome sites.
Efimova, N., Grimaldi, A., Bachmann, A., Frye, K., Zhu, X., Feoktistov, A., Straube, A. and Kaverina, I. (2014)
Podosome-regulating kinesin KIF1C translocates to the cell periphery in a CLASP-dependent manner.
Journal of Cell Science 127: 5179-5188. doi:10.1242/jcs.149633
tip tracking complications
In this paper we demonstrate that CLASPs modify the EB binding site at the microtubule lattice. Ben and Daniel demonstrate the removal of the EB binding site from the microtubule lattice through CLASPs using TIRF-based in vitro reconstitution experiments.
Grimaldi, A.D., Maki, T., Fitton, B.P., Roth, D., Yampolsky, D., Davidson, M.W., Svitkina, T., Straube, A., Hayashi, I. and Kaverina, I. (2014)
CLASPs are required for proper microtubule localization of end-binding proteins.
Developmental Cell, 30, 343-352.
In this paper we describe a computational framework to analyse and classify cell morphology. The approach uses a machine learning process based on the calculation of a shape similarity matrix and low-dimensional representation of cell shapes using diffusion map. We show that this approach is suitable for the highly variable cell shapes observed in migrating epithelial cells.
Jefferyes, S.D.R., Epstein, D.B.A., Straube, A., Rajpoot, N.M. (2013)
A novel framework for exploratory analysis of highly variable morphology of migrating epithelial cells.
Conf Proc IEEE Eng Med Biol Soc. 2013 Jul;2013:3463-3466, doi:10.1109/EMBC.2013.6610287
[link] [pubmed abstract]
composite microtubule binders
In this paper we demonstrate that TACC3 and clathrin form a composite microtubule binding site. Daniel and Anne did in vitro microtubule binding experiments of TACC3 and clathrin in the presence of AuroraA kinase to demonstrate the phosphorylation-dependent binding of the complex to microtubules.
Hood, F.E., Williams, S.J., Burgess, S.G., Richards, M.W., Roth, D., Straube, A., Pfuhl, M., Bayliss, R. & Royle, S.J. (2013)
Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding.
J. Cell Biol. 202:463-78
In this paper we investigate the flexural rigidity of microtubules that have been stabilised by Taxol, non-hydrolysable GTP analogs or two different microtubule-associated proteins, tau and MAP4. We find that Taxol and GTPγS make microtubules more flexible, while tau and GMP-CPP make them stiffer. We show that combinations of these stabilisers do not have additive effects, but rather one stabiliser dominates the mechanical properties. MAP4 is not changing rigidity per se, but limits the variability of rigidity between microtubules.
Hawkins T.L., Sept D., Mogessie B., Straube A. and Ross J.L. (2013)
Mechanical Properties of Doubly Stabilized Microtubule Filaments.
Biophysical Journal 104: 1517-1528
[link to pdf] [pubmed abstract][request pdf]
>> see all publications here
Anne Straube | PI
After a Diploma in Biochemistry and Molecular Biology from the University of Hamburg, Germany, I joined the lab of Gero Steinberg at the Ludwig Maximilian University in Munich and later at the Max Planck Institute for Terrestrial Microbiology in Marburg. Fo my PhD project I studied the microtubule cytoskeleton in the fungus Ustilago maydis, concentrating in particular on the dynamic re-organisation of microtubules during the cell cycle and on intracellular transport by the molecular motors dynein and kinesin, which carry traffic along microtubules. I then moved as a Postdoctoral Fellow of the Emmy Noether programme of the German Science Foundation (DFG) to the Wellcome Trust Centre for Cell Biology in Edinburgh, where I worked with Andreas Merdes on the microtubule cytoskeleton in differentiating muscle cells. In 2007, after three years in Edinburgh, I started my own lab at the Marie Curie Research Institute (MCRI) in Oxted, Surrey. When the MCRI closed in 2010, I moved with my colleagues Rob Cross and Andrew McAinsh to the University of Warwick to found the Centre for Mechanochemical Cell Biology.
Daniel Roth | research assistant | funded by Wellcome Trust
EB proteins are the master integrators of the plus tip network. I study the biochemical differences between EB1, EB2 and EB3 and contribute to collaborative projects that require my expertise in in vitro microtubule dynamics assays.
Jack Chen | Postdoctoral Fellow | funded by Leverhulme Trust
I study microtubule tip tracking transport using biochemical in vitro reconstitution assays.
Clare Garcin | Postdoctoral Fellow | funded by Wellcome Trust
I study microtubule organisation in differentiating muscle cells.
Paula Esquivias | Postdoctoral Fellow | funded by Wellcome Trust
I study microtubule-associated protein 4.
Jonathan Brandt | PhD student | funded by a WMS Scholarship
I study the changes in the host cytoskeleton upon infection with Marek's disease virus. In particular, I aim to understand the non-catalytic role of Us3 kinase on actin dynamics.
Alexander Zwetsloot | PhD student | funded by the MRC DTP
I study the bidirectional transport mediated by KIF1C and dynein.
Lewis Mosby | PhD student | funded by the Leverhulme Trust
I am modelling tip tracking transport.
Youchao Chen | PhD student | funded by a Chancellor's International Scholarship
I study KIF1C-dependent transport in neurons.
Julia Schander | Lister Institute summer student | funded by the Lister Institute of Preventive Medicine
I am engineering inhibitable kinesin motors.
Nida Siddiqui | Postdoctoral Fellow | 2018-2019
I studied Kif1C activation and force generation.
I am currently working for AstraZeneca in Cambridge.
Manas Chakraborty | Postdoctoral Fellow | 2016-2019
I studied microtubule self-organisation using biochemical in vitro reconstitution assays.
I am currently back in India and looking for faculty positions.
Mohammed Abdelsamea | Postdoctoral Fellow | 2018
I developed image analysis software.
I moved on to bea postdoc at Nottingham University and I am now a senior lecturer at Birmingham City University.
Gaëlle Letort | Software Developer | 2016
I developed image analysis routines to segment and measure microtubules in 3D superresolution images of human skeletal muscle cells.
I am currently a postdoc at the Institute Curie in Paris, France. [web]
I have a PhD in Computer Science and experience as a software developer with a specialty in human-computer interaction. I developed user-friendly software packages to analyse microtubule dynamics and cell shape.
I am currently senior scientific developer at zfx-innovation in Gargazzone, Italy. [web]
Ulrike Theisen | postdoctoral research fellow | 2008 - 2012
Kinesin motors perform the long-distance transport towards the plus end of microtubules. Thus kinesins are likely to be responsible for delivering polarity factors to the cell edges. I have identified Kif1C as the transporter for rearward transport of integrins in migrating cells and shown that this transport supports tail stability and rear drag, a mechanism important for directionally persistent cell migration in the absence of external guidance cues (Theisen et al., Dev Cell 2012).
I am currently a Marie Curie Fellow at Technische Universität Braunschweig and study neuronal migration in Zebrafish. [web]
Mike Downey | research associate | 2012-2013
I am a chemist and computer scientist by training and applied my expertise in semiautomatic image analysis to study how dynamic microtubules regulate cell shape and directional-persistent migration.
I moved on to be a postdoc in Till Bretschneider's group at the Warwick Systems Biology Centre and develop software tools for studying 3D cell shape dynamics.
I am now a software developer / data analyst in Birmingham.
Gokhan Tut | PhD student | 2015 - 2018
I studied the intracellular transport of Marek's Disease Virus.
I am currently a postdoc with Paul Moss at the Institute of Immunology and Immunotherapy, University of Birmingham.
Nida Siddiqui | PhD student | 2014 - 2018
I studied the autoinhibition mechanisms of Kif1C and its activation by PTPN21 (Siddiqui et al. Nat Comms 2019).
I stayed on as a postdoc in the Straube lab.
Alice Bachmann | PhD student | 2013 - 2017
I studied the mechanisms by which the kinesin Kif1C promotes the formation of podosomes in vascular smooth muscle cells (Efimova et al. J Cell Sci 2014) and identified PTPN21 as an activator of KIF1C-dependent intracellular transport (Siddiqui et al. Nat Comms 2019).
I am currently working for BioRad in Oxford.
Ben Fitton | PhD student | 2011 - 2016
Microtubule dynamics is characterised by phases of continuous growth and shrinkage and stochastic switches between these phases. I studied the transitions between growth and shrinkage phases, catastrophes and rescues, in detail using in vitro reconstitution of microtubule assembly/disassembly in the presence of EB proteins to detect the GTP cap.
I am currently on a civil service fast track apprenticeship applying my mathematical modelling skills to new kinds of problems.
Sam Jefferyes | PhD student | 2011-2015
Migrating cells adopt a variety of different shapes, which are determined by the cytoskeleton in response to the extracellular substrate. I developed machine learning algorithms for the unbiased quantification of cell morphology. I further analysed cell shape changes of migrating cells and developed models to predict cell behaviour from cell shape data alone.
I am currently software developer at quantumkdb in London - Glasgow - Ireland. [web]
Binyam Mogessie | PhD student
I investigated the role of MAP4 in cell division and muscle cell differentiation. I revealed a role for MAP4 in limiting force generation by cortical dynein in mitotic cells. This is important for spindle positioning (Samora, Mogessie et al., Nature Cell Biol 2011). I have also identified a novel MAP4 isoform required for the formation of antiparallel microtubule arrangement in differentiating muscle cells (Mogessie et al. eLife 2015).
I was a postdoc with Melina Schuh at the MRC Laboratory of Molecular Biology in Cambridge and I am now a group leader at the University of Bristol. [web]
Hannah Gay | part-time technician | 2016
I tried to generate homologous repair constructs and guideRNAs for CRISPR/Cas9-mediated labelling of endogenous cytoskeleton proteins with fluorescent markers.
I am now a PhD student in Birmingham.
Ana Clark | part-time technician | 2015
I performed 2D/3D cell migration assays with human breast cancer cells.
I am now a PhD student in Oxford.
Zoe Redshaw | part-time technician | 2014
I selected stable human breast cancer cell lines.
I am now a postdoc in Nottingham.
Hannah Smith| Rotation student Midlands Integrative Biosciences Training Programme | 2019
I studied the transport of Marek's disease virus.
I am now undertaking my PhD at Leicester.
Alice Haworth| Rotation student Midlands Integrative Biosciences Training Programme | 2017
I studied KIF15 localisation throughout the cell cycle.
I am now undertaking my PhD at Leicester.
Ainur Kakpenova | MSc by research student | 2014 - 2015
I studied spatial microtubule dynamics regulation in migrating cells.
I am now back at Nazarbayev University in Kazakhstan.
Jacopo Credi | Erasmus Mundus MSc in Complex Systems Science | 2015
I studied swarming behaviour of migrating cancer cells.
I am now undertaking the second year of my MSc at Ecole Polytechnique in Paris.
Alina Finch| Rotation student Midlands Integrative Biosciences Training Programme | 2015
I studied kinesin functions in cell migration.
I am now undertaking my PhD at Leicester.
Alexandra Matthews| Rotation student Midlands Integrative Biosciences Training Programme | 2015
I studied kinesin functions in cell migration.
I am now undertaking my PhD at Birmingham.
Justyna Szyroka | Rotation student Midlands Integrative Biosciences Training Programme | 2015
I studied changes in cell shape and motility of chicken embryonic fibroblasts upon infection with Marek's disease virus.
I am now undertaking my PhD at Warwick.
Diana Sifuentes Munch | Erasmus Mundus MSc in Complex Systems Science | 2014
I analysed correlations between cell shape and cell migration behaviour.
I am now undertaking the second year of my MSc at Ecole Polytechnique in Paris.
Jessica Talbot | MSc in Complexity Science | 2014
I worked with Stefan Grosskinsky (Warwick Complexity Centre) to simulate cell migration and division on a 2D surface with a sequential lattice model.
I am now undertaking the second miniproject for my MSc.
Lewis Baker | MSc student MOAC | 2014
I studied actin dynamics in migrating cells and the correlation with growing microtubule ends in proximity of the cell cortex.
I am now a PhD student.
Scott Clarke | MSc student MOAC| 2014
I purified tubulin from human cells.
I am now a PhD student with Mohan Balasubramanian.
Rozita Adib | Rotation student Midlands Integrative Biosciences Training Programme| 2014
I studied the effect of EML1 depletion on microtubule asymmetry in migrating cells.
I am now a PhD student with Andrew Fry at Leicester University.
Harriet Bell | MSc student Scientific Research and Communication| 2013
I studied the kinesin Kif1C and its interactors to find out how Kif1C transports vesicles along microtubules. In addition to my research I worked part time with the children's cancer team at the Cancer Research UK Clinical Trials Unit, University of Birmingham.
Nikita Nicholls | MSc student Systems Biology | 2012
I studied kinesins in spindle positioning.
I am now a PhD student in the Midlands Integrative Biosciences Training Partnership.
Sarah Cosgriff | MSc student Systems Biology | 2011
I studied cell shape changes and actin dynamics at the cell front in migrating cells.
I am now administrator at STEMNET and work at the University of Birmingham's STEM Centre to coordinate work with schools in Birmingham and Solihull.
Robert Lockley | MSc student Systems Biology | 2011
I studied the directionality of microtubule assembly in migrating cells.
I am now a PhD student with Graham Ladds and Till Brettschneider at the University of Warwick.
Charlotte Carroll | rotation student Life Sciences PhD | 2011
I studied the competition between EB1, EB2 and EB3 at the microtubule plus end in vitro.
I am now a PhD student with Lorenzo Frigerio at the University of Warwick.
Harold Moyse | MSc student Systems Biology | 2011
I studied the migration of differentiating muscle cells.
I am now a PhD student with Neils Evans and Daniel Zehnder the University of Warwick.
Rachel Sheldon | MSc student MOAC | 2011
I studied the microtubule binding properties of EB proteins.
I am now a PhD student with Hugo van den Berg, Anatoly Shmygol and Andy Blanks at the University of Warwick.
| MSc student Systems Biology | 2010
I studied cell behaviour during contact inhibition of locomotion.
I am now a PhD student with Yuriy Pankratov at the University of Warwick.
Samia Mohammed | BSCB summer student | 2019
I studied microtubule bundling by dynein.
I am now completing my degree in biomedical sciences at Birmingham City University.
Elly Straube | Lister Institute summer student | 2018
I engineered and characterised inhibitable Kif1C constructs.
I am now completing my degree in process engineering at the TU Dresden.
Meghana Kumar | Lister Institute summer student | 2016
I studied the regulation of microtubule dynamics by Kif1C.
I am now an MRes student at KCL.
Nida Siddiqui | Lister Institute summer student | 2014
I isolated tubulin from different sources rich and low in posttranslational modifications.
I was a PhD student and I am nowa postdoc in the Straube lab.
Luke Edwards | summer student | 2013
I spent my summer in the cold room to establish single-step purification of human tubulin using an affinity column with TOG domains.
I am now a BSc student at Bristol University.
Zainab Rahil | summer student | 2012
I analysed microtubule directionality and orderliness in muscle cells (Mogessie et al. eLife 2015).
I am now a PhD student in the Leckband group at the University of Illinois.
Natalia Wasiluk | Erasmus student | 2010
I spent a training internship in my final year as an undergraduate in the Straube lab and established TIRF-based in vitro microtubule plus end tracking assays using EB1, EB2 and EB3 (Roth et al., J Cell Sci 2019).
I am now working in the University of Cambridge Metabolic Research Laboratories.
Agnieszka Skalecka | summer student | 2008
As a summer student at the Marie Curie Research Institute, I characterised EB3-specific interacting proteins.
I am now a PhD student in Jacek Jaworski's lab at the IIMCB in Warsaw, Poland [web] [email]
Andrew McAinsh | CMCB | Warwick
Andrew and I collaborate since I started my lab at the Marie Curie Research Institute. We share an interest in live cell imaging and what microtubules do when they arrive at subcellular structures, the kinetochore or the cell cortex. We have successfully combined our expertise investigating MAP4 and CLASP1 and dynein in mitotic spindle positioning (Samora et al., Nature Cell Biology, 2011).
Jenny Ross | UMass | Amherst
Jenny and I first met at the "Microtubule Dynamics" workshop I organised at the Marie Curie Research Institute. We met later at the EMBL Microtubule conference and decided to do some work together. Jenny has helped us with dynein gliding assays to prove the direct role of MAP4 in controlling dynein force generation (Samora et al., Nature Cell Biology, 2011) and we have provided her lab with MAP proteins to study the mechanical properties of microtubules in the presence of various microtubule stabilisers (Hawkins et al., Biophysical Journal, 2013).
Andrew Fry and Richard Bayliss | Leicester University
Andrew, Richard and I collaborate on the structure and function of EML proteins, microtubule-associated proteins that form oncogenic fusion proteins with tyrosine kinases (Richards et al., Biochem J 2015; Adib et al., Science Signal 2019).
Irina Kaverina | Vanderbilt University
Irina and I share an interest in how dynamic microtubules control directional cell migration. We have recently documented our ideas on this in a review article (Kaverina & Straube, Seminars Cell Dev Biol, 2011). We held a joint lab retreat in May 2012 and currently collaborate on the role of microtubules and the motor Kif1C in podosome formation (Efimova et al., J Cell Science 2014; Siddiqui at al., Nature Comms 2019) and on the microtubule plus tip complex (Grimaldi et al., Developmental Cell 2014).
Ekkehard Straube | Physik | Universität Halle
My father is a (retired) theoretical physicist who applies his knowledge of polymer physics to the analysis of our cell migration tracks. He has contributed a directional persistence analysis program to our recent paper on the role of Kif1C-mediated stabilisation of trailing adhesions in directed cell migration (Theisen et al., Developmental Cell, 2012).
Nasir Rajpoot | Computer Sciences | Warwick
Nasir and I co-supervised a systems biology PhD project into the analysis of shape variations of migrating cells with the aim to understanding the relationship of cell shape and migratory behaviour, to explore and quantify morphological phenotypes and shape fluctuations over time (Jefferyes et al., Conf Proc IEEE Eng Med Biol Soc, 2013).
Steve Royle | CMCB | Warwick
Since becoming office neighbours, Steve and I have started to collaborate on aspects of clathrin's microtubule binding properties (Hood et al., Journal of Cell Biology, 2013). In the future we want to exploit our complementary expertise to understand the handing over of vesicles from clathrin-mediated endocytosis to microtubule-based transport.
Project Grant | Leverhulme Trust
How microtubule plus tip trackers couple polymer assembly to cargo transport
Co-Applicants: Marco Polin and Robert Cross
£256,434 - 2017 to 2020
Investigator Award in Science | Wellcome Trust
Self-organisation of acentrosomal microtubule arrays
£943,108 - 2016 to 2021
Lister Research Prize | The Lister Institute of Preventive Medicine
Microtubule-actin crosstalk in cell migration
£200,000 - 2014 to 2019
Warwick Quantitative Biomedicine Programme Seed Fund | Wellcome Trust / University of Warwick
Microtubule control of cell and tissue morphology
£25,000 - 2015 to 2016
PhD studentship | British Heart Foundation
Kinesin-control of podosome formation of vascular smooth muscle cells
£105,584 - 2013 to 2016
Programme Grant | Marie Curie Cancer Care
Mechanisms of directional cell migration and differentiation
£630,000 - 2009 to 2013
Core Funding | Marie Curie Cancer Care
Cytoskeletal Organisation Laboratory
2007 to 2009
Brazil Partnership Fund | Santander / University of Warwick
Collaboration with Deborah Schechtman - Phosphoregulation of tubulin assembly
£8,700 - 2014 to 2016
Collaboration Fund | EPSRC Grand Challenge network "Understanding the Physics of Life"
Collaboration with Massimo Antognozzi - Lateral Motion Force Microscopy of microtubule dynamics
£2,405 - 2014
Research Development Fund | University of Warwick
Breast cancer cell migration
£9,000 - 2014 to 2015
Strategic Partnership Fund | University of Warwick
Collaboration with Irina Kaverina (Vanderbilt) - Microtubules in directional migration
£10,000 - 2011 to 2012
Joint Warwick-QMUL Awards for Collaborative Research
Collaboration with Ann Wheeler (QMUL) - Superresolution imaging of +TIPs
£29,123 - 2012 to 2013
>> Microtubule brick modelling
Our school outreach programme includes building models of intracellular molecular machines from construction toy bricks. If you are interested in hosting a visit from us, please do get in touch.
top | Second generation brick model of a polymerising microtubule end, alpha-tubulin in black, GTP-bound beta-tubulin in yellow, GDP-bound beta-tubulin in red.
left | My son has been testing my brick collection and we built: a DNA double helix, a mitotic cell picture and a viral capsid. So it's not just microtubules...
>> Pictures from the exhibition of our Art & Science project "The Fox Got You" with Françoise Sergy in the Oxford Botanic Garden from 4th July to 31st August 2015.
left | Anne explains the wonders of microtubule research and how microtubule-targeting drugs work - at the public event during the exhibition at the Oxford Botanic Garden.
Impressions from the exhibition describing 5 medicinal plants and their use in research and the clinic.
Please visit the website documenting the entire project including the laboratory visits. There is a lot to learn how to grow the plants that make important medicines and to be impressed by the MRC units giant LEGO enzyme!
more coming soon...
In the future you will find here:
>> Instructions how to build a microtubule from LEGO bricks and images of our first and second generation microtubule models.
>> Plain english explanations of our research projects.
There are a number of opportunities to join our eggceptional team of scientists. We are an interdisciplinary and international group, united by the love of science, our famous cakeclub and any other eggcellent morale boosting activities.
Lister Institute-funded summer studentship
One summer studentship for 10 weeks in Summer 2020 is available in my lab. You will work on a quantitative imaging project and receive a stipend of £235 per week. If you cannot imagine a more exciting summer than one spent in the lab, please send me a CV, a personal statement and one academic reference before 1st April 2020.
PhD students and postdocs
We are always looking for excellent students and postdocs to apply for scholarships and fellowships and bridging funds are available to strong candidates with matching research interests. If you are motivated to work in an interdisciplinary environment, have a background in biology, physics, maths or computer science and are enthusiastic about our research, then please get in touch with your CV and a brief statement of research interests.