dynamics research summary

We are interested in understanding the physical division of one cell into two. This process, referred to as cytokinesis, requires an actomyosin based contractile ring in several eukaryotes and a tubulin based Z-ring in bacteria. Our eukaryotic work uses fission yeast, and involves a combination of genetics, live cell imaging, in vitro reconstitution and other biophysical approaches to understand how the actomyosin ring assembles in space and time and how it constricts. For work on prokaryotic cytokinesis, we employ a synthetic reconstitution approach, in which we express bacterial cytoskeletal proteins in fission yeast and study their properties using high resolution imaging and mutagenesis. We believe new insight will be gained from comparative analyses of eukaryotes and prokaryotes that can be transferred and may pave the way for translation.


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springlatest: Srinivasan, R. & Balasubramanian, M.K.(2014)
Bacteria spring a surprise.

eLife, 3: e03435.

pombelatest: Tao, E.Y., Calvert, M. & Balasubramanian, M.K.(2014)
Rewiring Mid1p-independent medial division in fission yeast

Current Biology, 24: 2181-8.


Chew TG, Huang J, Palani S, Sommese R, Kamnev A, Hatano T, Gu Y, Oliferenko S, Sivaramakrishnan S, Balasubramanian MK. Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction.
J Cell Biol. 2017 Jun 27. pii: jcb.201701104. doi: 10.1083/jcb.201701104.


Palani, S., Chew, TG, Srinivasan, R., Kamnev, A., Mishra, M., Sevugan, M., Chapa-y-Lazo, B., Gayathri, P., and Balasubramanian, M.K. (2017) Motor activity dependent and independent functions for Myosin II in Cytokinesis.
Current Biology 12:751-757


Zambon, P., Palani, S., Kamnev, A., Balasubramanian, M.K. (2017). Myo2p is the major motor involved in Actomyosin Ring Contraction in S.pombe.
Current Biology 27:R99-100.


Huang, J., Chew, TG, Gu, Y., Palani, S., Kamnev, A., Carter, N., Martin, D, Cross, RA., Oliferenko, S., Balasubramanian,M.K. (2016) Curvature induced expulsion of actomyosin bundles during cytokinesis in fission yeast.
eLife. (http://dx.doi.org/10.7554/eLife.21383)


Sethi K, Palani S, Cort├ęs JC, Sato M, Sevugan M, Ramos M, Vijaykumar S, Osumi M, Naqvi NI, Ribas JC, Balasubramanian M. (2016). A New Membrane Protein Sbg1 Links the Contractile Ring Apparatus and Septum Synthesis Machinery in Fission Yeast.
PLoS Genet. 12(10):e1006383.


Cheffings, T., Burroughs, N.J., and Balasubramanian, M.K. (2016). Actomyosin ring formation and tension generation in eukaryotic cytokinesis.
Current Biology. 26: R719-37.


Tao, E.Y., Calvert, M.E., and Balasubramanian, M.K. (2014) Rewiring Mid1p-Independent Medial Division in Fission Yeast.
Current Biology 22: 891-9


Mishra, M., Kashiwazaki, J., Tagagi, T., Huang, Y., Srinivasan, R., Balasubramanian, M.K., and Mabuchi, I. (2013) In Vitro Contraction of Cytokinetic Ring Depends on Myosin II but not on Actin Dynamics.
Nature Cell Biology 15:853-859


Huang, J., Huang, Y., Yu, H., Padmanabhan, A., Subramanian, D., Tao, Y., Thadani, R., Tang, X., Wedlich-Soldner, R., and Balasubramanian, M.K. (2012) Non-medially assembled F-actin cables incorporate into the actomyosin ring in fission yeast.
Journal of Cell Biology 199: 831-847


Srinivasan, R, M Mishra, L Wu, Z Yin and MK Balasubramanian (2008) The bacterial cell division protein FtsZ assembles into cytoplasmic rings in fission yeast.
Genes & Development 22:1741-1746