dynamics research summary

We are interested in understanding the physical division of one cell into two. This process, referred to as cytokinesis, requires an actomyosin based contractile ring in several eukaryotes and a tubulin based Z-ring in bacteria. Our eukaryotic work uses fission yeast, and involves a combination of genetics, live cell imaging, in vitro reconstitution and other biophysical approaches to understand how the actomyosin ring assembles in space and time and how it constricts. For work on prokaryotic cytokinesis, we employ a synthetic reconstitution approach, in which we express bacterial cytoskeletal proteins in fission yeast and study their properties using high resolution imaging and mutagenesis. We believe new insight will be gained from comparative analyses of eukaryotes and prokaryotes that can be transferred and may pave the way for translation.

 

> lab home page is here

 

springlatest: Srinivasan, R. & Balasubramanian, M.K.(2014)
Bacteria spring a surprise.

eLife, 3: e03435.
[link]

pombelatest: Tao, E.Y., Calvert, M. & Balasubramanian, M.K.(2014)
Rewiring Mid1p-independent medial division in fission yeast

Current Biology, 24: 2181-8.
[link]

 

Tao, E.Y., Calvert, M.E., and Balasubramanian, M.K. (2014) Rewiring Mid1p-Independent Medial Division in Fission Yeast.
Current Biology 22: 891-9

 

Mishra, M., Kashiwazaki, J., Tagagi, T., Huang, Y., Srinivasan, R., Balasubramanian, M.K., and Mabuchi, I. (2013) In Vitro Contraction of Cytokinetic Ring Depends on Myosin II but not on Actin Dynamics.
Nature Cell Biology 15:853-859

 

Huang, J., Huang, Y., Yu, H., Padmanabhan, A., Subramanian, D., Tao, Y., Thadani, R., Tang, X., Wedlich-Soldner, R., and Balasubramanian, M.K. (2012) Non-medially assembled F-actin cables incorporate into the actomyosin ring in fission yeast.
Journal of Cell Biology 199: 831-847

 

Srinivasan, R, M Mishra, L Wu, Z Yin and MK Balasubramanian (2008) The bacterial cell division protein FtsZ assembles into cytoplasmic rings in fission yeast.
Genes & Development 22:1741-1746