dynamics research summary

The Sampath laboratory is interested in understanding the mechanisms that specify the embryonic axes, and in elucidating the molecular functions of factors that participate in these processes. In particular, we are investigating the roles and regulation of maternally deposited RNA and protein factors in early embryonic development, using the zebrafish as a model system. We use a combination of live imaging, biochemistry, genetics, genomic, and embryological approaches. Our current research focuses on three major themes: 1) Microtubule based transport and localization of maternal RNAs with functions in embryonic axis determination, 2) Functions and regulation of non-coding maternal nodal RNA and 3) Regulation of nodal signaling.



> lab home page is here


fishlatest: Quach, H.N., Tao, S., Vrljicak, P., Joshi, A., Ruan, H., Sukumaran, R., Varshney, G.K., LaFave, M.C., Ds Screen Team, Burgess, S.M., Winkler, C., Emelyanov, A., Parinov, S., & Sampath, K. (2014)A Multifunctional Mutagenesis System for Analysis of Gene Function in Zebrafish.
G3 (Bethesda), 5: 1283-99. [link]


Kumari P, Gilligan PC, Lim S, Tran LD, Winkler S, Philp R, Sampath K. (2013) An essential role for maternal control of Nodal signaling.
Elife. 2013 Sep 10;2:e00683.


Lim S, Wang Y, Yu X, Huang Y, Featherstone MS, Sampath K. (2013) A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases.
Genome Biology14:R69.


Tran, L.D., Hino, H., Quach, H., Lim, S., Shindo, A., Mimori-Kiyosue, Y., Mione, M., Ueno, N., Winkler, C., Hibi, M. and Sampath, K. (2012) Dynamic Microtubules at the Vegetal Cortex Predict the Embryonic Axis in Zebrafish.
Development 139: 3644-3652


Gore, A., Maegawa, S., Cheong, A., Gilligan, P., Weinberg, E., and Sampath, K. (2005) The zebrafish dorsal axis is apparent by the four-cell stage.
Nature 438: 1030-1035